Project Summary This supplement application to the parent grant R00NS117804 is to support Ms. Iris Farnum, a postbaccalaureate research assistant in her first year of training. Iris will receive direct mentoring from me (Manideep Chavali, PI) and will be co-mentored by Dr. Ben Emery. This supplement will enhance Iris's training, enabling her to focus on her research and acquire the technical and professional skills necessary to become a competitive graduate school applicant and earn a Ph.D. in neuroscience. The research training plan has two main components. Firstly, Iris will develop technical expertise in skills such as single molecule fluorescent in situ hybridization (smFISH), immunohistochemical analysis of human and rodent neonatal brain injury specimens This will also encompass training in confocal microscopy, image acquisition, and data analysis. Secondly, she will gain expertise in developmental neuroscience concepts as she characterizes the functional consequences of hypoxic injury on oligodendroglial lineage cells and white matter maturation. For this purpose, she will also receive training in molecular biology techniques, including protein isolation and expression analysis, RNA isolation, and expression analysis. She will also become proficient in isolation and aseptic culture of primary oligodendroglial and ex vivo cerebellar slice cultures techniques. Additionally, Iris will receive training in data analysis, statistical methods, and effectively communicating research findings.