Abstract This application seeks supplement support to replace a frequently failing and costly to maintain chromatography system (FPLC, Amersham Pharmacia) from 2010 with an up to date FPCL instrument. Virtually all work performed in our lab requires proteins purified by a FPLC system. Our current system is more than 10 years old, no longer supported by the manufacturer, frequently failing and incurring high costs of repair from third party suppliers. Since purified proteins are crucial for our work, we rely on this type of instrumentation and request a new instrument (Akta pure 25 L) with essentially the same capabilities as the existing instrument.