PROJECT SUMMARY The Arkansas Integrative Metabolic Research Center (AIMRC) is a Phase I COBRE at the University of Arkansas at Fayetteville (04/01/2021-02/28/2026; PI: Dr. Kyle P. Quinn). The overall scientific objective of the AIMRC is to investigate the role of cell and tissue metabolism in disease, development, and repair through research involving advanced imaging, bioenergetics, and data science. The long-term objective of the AIMRC is to establish a sustainable interdisciplinary research center that can support biomedical research at the U of A and grow the emerging strengths in metabolic research on the University of Arkansas campus. The Bioenergetics Core currently assists researchers with measurements of metabolic flux at the cell, isolated tissue, and whole animal levels. Through the acquisition of an LC/Q-TOF mass spectrometer, this supplement will add the first and only metabolomics capabilities on our campus. The comprehensive measurement of metabolites through the LC/Q- TOF system will enable researchers to identify changes in flux through specific metabolic pathways and will complement other sophisticated core instruments (including three Agilent Seahorse analyzers, an Oroboros O2 respirometer, BioTek Cytation 5 Imaging Multimode analyzer, and a Promethion metabolic cage system). Collectively this suite of equipment will allow the AIMRC to provide valuable metabolic information under different disease conditions at the molecular, cellular, tissue, and whole-body levels. Acquisition of the LC/Q-TOF mass spectrometer will also help in obtaining a clear description of metabolites across a diverse set of biomedical applications, and would enable the analysis of key biochemical pathways altered in functional genomics and proteomics studies. More, importantly, the LC/Q-TOF will provide a unique resource to our campus and region. It will be highly beneficial for the research proposed by current AIMRC COBRE research project leaders and recruited new faculty who will be candidates for future advancement to research project leaders.