ABSTRACT We are applying for an NIH Supplement fund to support our current R15 grant, focusing on the epigenetic regulation of chromatin by the Keap1-Nrf2 xenobiotic response signaling pathway in Drosophila. The purpose of this application is to acquire a fluorescence microscope. The Keap1-Nrf2 signaling pathway is essential for oxidative and xenobiotic responses and is related to many diseases especially cancer. To understand the impact of environmental toxins on development and health at the molecular level, my laboratory investigates the molecular mechanisms and biological functions of the Keap1-Nrf2 oxidative and xenobiotic response pathway in Drosophila. Supported by the current R15 fund, we aim to characterize the molecular and biological interactions between dKeap1/CncC and chromatin remodeling proteins, including lamin, actin, NURF, and JIL-1. Additionally, we plan to identify developmental genes directly targeted and regulated by dKeap1 in a redox-independent manner. Furthermore, we will study the formation and function of dKeap1-induced condensates. Finally, we will test the hypothesis that dKeap1 and/or CncC mediate developmental adaptations to xenobiotic stimuli through epigenetic regulation of chromatin architecture. This research is also expected to enhance undergraduate education in both the classroom and laboratory settings. All these research plans involve numerous fluorescence imaging assays, such as immunostaining and live imaging. Therefore, an advanced fluorescence microscope is essential for the ongoing projects in our lab. Unfortunately, the only fluorescence microscope shared by our department is outdated and dysfunctional. Currently, we are utilizing a campus-shared microscope located in a different building. Our lab urgently requires a fluorescence microscope that is easily accessible. Additionally, the acquisition of this equipment will significantly enhance the research capabilities of other faculty members within the Biology Department at the University of Minnesota Duluth.