Sepsis is a global health problem and is defined as organ dysfunction as a result of an altered immune response to an infection. With such a large burden of mortality worldwide, better diagnostics and treatments are necessary for this disease. This application will focus on monitoring the immune response during severe infections that lead to sepsis. Current clinical practices rely on traditional blood culture to assess infections and immunoglobulin activity. Here we propose to use deep RNA sequencing as a means to evaluate the mechanism of immunosuppression in sepsis patients as a result of severe infection over time to better characterize immunosuppression in sepsis patients, allowing clinicians to more effectively treat antimicrobial resistant infections.