Summary The purpose of the proposed research is to facilitate learning experiences in a two-year post-doctoral. training experience, connecting the intersection between learning novel imaging algorithms to risk stratify individuals with high-risk Peripheral Artery Disease (PAD) as well as their clinical validation and association with clinically relevant outcome measures. In addition, this grant is also looking to provide opportunities for underrepresented background post-doctoral, Dr. Tapia. She will be a part of the development of this project and will be supported with the tools and opportunities to learn this scientific/research pathway with the principal goal of empowering her for an independent project in the future. Integration of professionals with diverse backgrounds is the way to create and develop research projects with the vision to provide better outcomes for the community. Peripheral Artery Disease (PAD) poses a significant growing public health concern, with millions worldwide affected by progressive functional decline. Association with other comorbidities such as Diabetes Mellitus (DM) creates a challenging approach diagnostically and treatment-wise. Our proposed research seeks to get a better understanding of how the pathophysiology and adaptations at the microvascular levels for PAD and DM are seen. With imaging dual isotope (82Rb/18F-LMI1195) hybrid Positron Emission Tomography (PET)/ Computerized Tomography (CT) these imaging techniques are great options for these patients, helping the future approach and treatment. Amis 1A, 1B, and 2 will be focused on understanding the technical aspects of PAD, as well as how the different imaging procedures will give us a better understanding of this condition. Aim 3 will be directed to validate the data using the PORTRAIT and SCOPE-CLI study designs. Data recollection will allow us to evaluate imaging protocols in a real-world population and correlate risk profile information with imaging characteristics validating the imaging protocols, while also generating further hypotheses. The proposed project represents the implementation of a new imaging protocol and generating benefits in the approach and treatment of this chronic condition, PAD. The training position will span over a period of 2 years, during which it will be involving a combination of the pre-clinical aspect in animal models, and then screening in human subjects. Accurate quality control measures in the image acquisition and data recollection period as well as the multidisciplinary collaboration will be needed to achieve high-quality outcomes and robust validation and translational potential. In conclusion, this proposal ambitions to enhance Dr. Tapia's learning experience by integrating new knowledge and research skills. It will also involve the development and implementation of imaging protocols for future approaches and treatment of PAD and DM.