# Chemical glycoproteomics

> **NIH NIH R01** · STANFORD UNIVERSITY · 2024 · $26,407

## Abstract

PROJECT ABSTRACT
Mucins are heavily glycosylated proteins that have been known to play roles in inflammation
and cancer with potential to be therapeutic targets for future treatments1. Probing the mucinome
is something that the Bertozzi lab has worked towards since 2002 in the form of mucin-type
O-glycoproteomics technology development. To build upon these accomplishments, I will work
towards the development of a new mucin-selective enrichment strategy for mass spectrometry
analysis of the “mucinome” by utilizing a novel proximity labeling approach6. To do this, I will
develop a StcE[E447D] mucinase with an iridium photocatalyst conjugate which will catalyze the
labeling of mucin domain containing proteins with a biotin probe which can then be enriched.
The conjugated StcE[E447D] mucinase will be validated utilizing mass spectrometry
glycoproteomics in prostate cancer cell models. Finally, I will also expand upon the existing
library of inactive mucinases by adding inactive mucinases with photocatalyst conjugates.

## Key facts

- **NIH application ID:** 11063454
- **Project number:** 3R01CA200423-21S1
- **Recipient organization:** STANFORD UNIVERSITY
- **Principal Investigator:** Carolyn Bertozzi
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $26,407
- **Award type:** 3
- **Project period:** 2002-08-01 → 2026-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11063454

## Citation

> US National Institutes of Health, RePORTER application 11063454, Chemical glycoproteomics (3R01CA200423-21S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/11063454. Licensed CC0.

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