# Development of Allogeneic CAR T Cell Therapy for a Functional Cure of HIV Infection

> **NIH NIH R01** · MASSACHUSETTS GENERAL HOSPITAL · 2024 · $165,664

## Abstract

Contact PD/PI: Allen, Todd M.
Abstract: Antiretroviral therapy (ART) dramatically reduces HIV-associated morbidity and mortality (1).
However, it is not a practical cure as eradication of HIV through ART alone is estimated to require over 60 years
of treatment (1, 2). Numerous studies support that HIV-specific T cell responses are critical for efficient targeting
and elimination of HIV infected cells that are the source of chronic infection (3-10). Unfortunately, viral escape
and a limited presence of functional virus-specific effector CD8+ T cells undermine the potency of these
responses in chronically infected individuals (11-17). As such, there is growing interest in the development of
novel immunotherapeutic approaches to target and eliminate HIV-infected cells to achieve viral
suppression in the absence of ART, a “functional cure”.
 Chimeric antigen receptor (CAR) T cell immunotherapies have demonstrated great promise against
blood cancers (18-20), and now also demonstrate the potential to mitigate HIV/SIV infection in rhesus macaques
(21, 22) and humanized mice (23-28). We recently showed that HIV-specific Dual CD4-based CAR T cells co-
expressing independent 4-1BB and CD28 costimulatory domains restrict HIV replication and reduce viral burden
in humanized mice (23). However, current limitations of using autologous T cells to derive CAR T cell products
(TCPs), including time-consuming and costly manufacturing, insufficient or dysfunctional patient-derived T cells,
and the inter-patient heterogeneity of TCPs, are barriers to their widespread application to human diseases.
Development of allogeneic TCPs derived from healthy human donors, could, however, provide an ‘off-the-shelf’
treatment option to overcome these hurdles, as well as accelerate the use of CAR T cell therapies (29-38).
Unfortunately, post-infusion elimination by the recipient’s immune system remains a major hurdle (39-41).
 Here we propose to leverage our expertise in CAR T cell biology (21, 23, 25, 26, 42, 43), base editing
(44-49), and a humanized mouse model of HIV infection (50-58) to develop an allogeneic CAR T cell therapy
against HIV. Building on our preliminary data applying efficient multiplex base editing to CAR T cells, we
hypothesize that both base editing approaches and identification of an optimal allogenic donor will enable the
development of an allorejection-resistant CD4-based CAR TCP with enhanced efficacy to eliminate HIV-infected
cells and suppress HIV in the absence of ART. To test this hypothesis, we propose the following specific aims:
Aim 1: Determine whether genetic modifications to allogeneic T cells can augment their in vivo
persistence.
Aim 2: Identify characteristics of allogeneic HIV-specific CD4CAR T cells that associate with enhanced
 persistence and antiviral efficacy.
Aim 3: Compare the in vivo HIV efficacy of allogeneic versus autologous HIV-specific CD4CAR T cells,
incorporating Aim 1 and 2’s signatures of improved allogeneic functionality.
...

## Key facts

- **NIH application ID:** 11075035
- **Project number:** 3R01AI170189-03S1
- **Recipient organization:** MASSACHUSETTS GENERAL HOSPITAL
- **Principal Investigator:** Christian L. Boutwell
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $165,664
- **Award type:** 3
- **Project period:** 2022-03-01 → 2027-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11075035

## Citation

> US National Institutes of Health, RePORTER application 11075035, Development of Allogeneic CAR T Cell Therapy for a Functional Cure of HIV Infection (3R01AI170189-03S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/11075035. Licensed CC0.

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