PROJECT SUMMARY Since any antimicrobial drug use has the potential to increase resistance, a key goal is to align antimicrobial drug use (AU) with the prescribing guidelines in veterinary settings. Awareness alone is inadequate to achieve concordance with prescribing guidelines since other factors, such as perceived pressure to prescribe by clients, hospital culture, and a lack of confidence in communicating with clients have been described as barriers to judicious use. Information is lacking on the impact of available educational resources to reduce veterinarian's intentions to prescribe antibiotics as well as their confidence that they can align their prescribing to AS principles. An area of identified antimicrobial overuse in companion animal medicine is canine acute diarrhea, for which up to 70% of veterinarians have been reported to prescribe or intend to prescribe metronidazole. These factors make it a crucial area for testing the impact of targeted resources designed to persuade veterinarians to reduce antimicrobial use. To foster antimicrobial stewardship in veterinary settings using training or education tools, we will use the Theory of Planned Behavior to quantify the knowledge, attitude, subjective norm (perceived social pressure regarding guidelines), and perceived behavioral control (perceived ability to follow the guideline) related to prescribing metronidazole for dogs with a common medical condition (i.e., acute diarrhea). We will explore whether a short educational video on antimicrobial stewardship or a summary of evidence-based guidance with accompanying citations can impact veterinarians' opinions on prescribing antibiotics for canine acute diarrhea and define characteristics of antimicrobial use resources that veterinarians identify as effective in changing their intention to reduce antimicrobial prescribing for canine acute diarrhea. The impact of these resources will be assessed using a cross-sectional survey of companion animal veterinarians in the United States randomized to 3 arms (no resource, educational video, summary of evidence-based guidance) followed by focus groups with participants drawn from each group to define which aspects of AS resources are effective in reducing veterinarians' intent to prescribe antimicrobial drugs. The outcome of these aims will facilitate a greater understanding of the potential impact of AS resources on aligning veterinary AU with AS guidelines and inform further research into the creation and implementation of resources to companion animal veterinarians to further the goals of antimicrobial stewardship.