# Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution

> **NIH NIH R35** · UNIVERSITY OF VIRGINIA · 2024 · $18,501

## Abstract

Abstract
We aim to greatly extend our existing studies in the proposed period, capitalizing on the great
advances in electron cryo-microscopy that have been due to improved direct electron detectors,
better software, and faster computers. The studies will include pili and flagellar filaments from
pathogenic bacteria, mating pili, type III and IV secretion systems, and archaeal viruses infecting
hosts living in the most extreme environments. In addition, we will continue studies of small
molecules and peptides that self-assemble into tubes and filaments, many with anticipated
biomedical applications. Our work on actin and why the sequence has been so highly conserved
will continue. By working on many disparate polymers, we expect to develop more general
methods that can be applied by others to helical polymers in biology. Since large numbers of such
polymers exist, ranging from cytoskeletal filaments to amyloid to helical viruses (such as Ebola)
the potential significance of this work is enormous and likely to have a great impact.

## Key facts

- **NIH application ID:** 11076053
- **Project number:** 3R35GM122510-08S1
- **Recipient organization:** UNIVERSITY OF VIRGINIA
- **Principal Investigator:** EDWARD H. EGELMAN
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $18,501
- **Award type:** 3
- **Project period:** 2017-05-01 → 2027-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11076053

## Citation

> US National Institutes of Health, RePORTER application 11076053, Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic Resolution (3R35GM122510-08S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/11076053. Licensed CC0.

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