# Characterization of iGL-VRC01 Memory B cell Responses in Mice

> **NIH NIH R01** · SCRIPPS RESEARCH INSTITUTE, THE · 2024 · $150,000

## Abstract

PROJECT SUMMARY
The behavior of B cell memory in mice and humans is an active area of research with implications for
traditional vaccine design and for the development of our engineered B cell vaccine (parent award). There
is data in mice supporting the idea that memory B cells have a very limited ability to re-enter germinal
centers. Alternatively, it has been shown that di?erent memory phenotypes exist with some showing a
preference for germinal center re-entry. We hypothesize that memory behavior, similar to naive cell
behavior, may also depend on immunogen a?inity, valency, and availability of T cell help. We will explore
these ideas by generating various memory phenotypes using in vitro B cell activation and adoptive transfer
to increase the frequency of antigen-specific memory in the B6 mouse model (which is normally very low).
We will then vaccinate using antigens with defined a?inities and valency’s to see if these factors impact
memory cell developmental fates. We would also like to immunize memory at di?erent times to see if
changing memory B cell surface markers are associated with altered cell behaviors in response to
vaccination.

## Key facts

- **NIH application ID:** 11076149
- **Project number:** 3R01AI165143-04S1
- **Recipient organization:** SCRIPPS RESEARCH INSTITUTE, THE
- **Principal Investigator:** James Even Voss
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $150,000
- **Award type:** 3
- **Project period:** 2021-07-01 → 2026-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11076149

## Citation

> US National Institutes of Health, RePORTER application 11076149, Characterization of iGL-VRC01 Memory B cell Responses in Mice (3R01AI165143-04S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/11076149. Licensed CC0.

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