Project Summary / Abstract A Sorvall LYNX 6000 superspeed centrifuge is requested to enable critical steps during protein isolation protocols that constitute an essential part of the research plan supported by grant 1R16GM145435-01A1. At a quoted cost of $76,200, the centrifuge together with a set of three rotors will facilitate the purification of the Plasmodium enzyme PfATP6 for activity inhibition assays. While this type of centrifuge allows for the processing of large sample volumes at low speeds, it also facilitates spins of smaller volumes at high speeds that create a relative centrifugal force of 100,000 x g. The latter type of spin is essential for parts of the PI’s research agenda and currently not possible at the PI’s institution since the chemistry department’s Beckman TL-100 highspeed centrifuge ceased to function last year and there is no other highspeed centrifuge on campus. Due to its age, the Beckman TL-100 model it is beyond repair because of the unavailability of spare parts. As a result, the PI is currently taking samples to the pharmacology department at UC Davis (20 miles away) in order to use a highspeed centrifuge there. However, these biweekly or monthly trips to UC Davis, each one requiring several hours of the PI’s time, are only a temporary solution and they also prohibit student training in the use of such an instrument. Thus, the acquisition of a superspeed centrifuge would have tremendous benefits for the project.