Enhanced Sensitivity and Quantitative Precision for Single Cell Proteomics

NIH RePORTER · NIH · R01 · $65,450 · view on reporter.nih.gov ↗

Abstract

Single cell proteomics (SCP) is rapidly emerging and can quantify > 1000 proteins per cell. Significant advances in instrumentation and sample preparation are making SCP more broadly accessible. Yet technical advances in data acquisition have not been paired with advances to computational tools. Algorithms for proteomics were designed and optimized on data from bulk proteomics, and are ill-suited for SCP data. Our preliminary research shows that data from SCP lack many features that are critical for current proteomics algorithms. We will dramatically improve accuracy and coverage of the single cell proteome through creation of the first-ever dedicated SCP search software. This will be coupled with an initiative to improve SCP peptide and protein quantification. These algorithmic improvements will be informed from a large corpus of SCP data, gathered and centralized into the first SCP data archive.

Key facts

NIH application ID
11088043
Project number
3R01GM147653-03S1
Recipient
BRIGHAM YOUNG UNIVERSITY
Principal Investigator
Samuel H Payne
Activity code
R01
Funding institute
NIH
Fiscal year
2024
Award amount
$65,450
Award type
3
Project period
2022-09-27 → 2026-05-30