Project Summary The success of our R35 has resulted in the implementation of the XCMS-METLIN online informatic platform, an effort that has been decades in the making. This technology, especially with the incorporation of METLIN’s MS/MS data, is causing explosive growth in the number of registered users, now over 50,000. To support this growing user base, we request support for a more advanced server system with the primary purpose to leverage this new breakthrough technology. This resource will remain freely available to the 50,000 plus registered users worldwide and has been previously supported by Scripps with over $200,000 in internal funding. This supplement will also help meet the strategic plan of the NIGMS MIRA grant program to support ambitious projects, move research into new directions, improve our research productivity, and effectively mentor the next generation of scientists. Our efforts have been extremely effective in establishing new scientists in metabolomics with new faculty at Yale University, Washington University, University of Colorado, Imperial College London, University of Lausanne, Montana State University, Colorado State University, as well as many others, and the research described here will continue to facilitate the mentoring environment important to the MIRA program. As a part of the research described herein, we request a supplement to support the acquisition of new computer server hardware for our new XCMS-METLIN platform, a groundbreaking technology that will impact the entire community. 1