ABSTRACT: Funded by the parental R01, 5R01GM146756, we have been studying the regulation of a novel family of short RNAs and their functions. These studies require us to prepare carefully size-selected cDNAs that are derived from short RNAs of specific lengths. This proposal is for an equipment supplement that will enable us to purchase a newly available piece of equipment, PippinHT, to more efficiently prepare size-selected cDNA libraries to advance the specific aims of the parental R01.