Project Summary This proposal is to purchase the Amersham ImageQuant 800 biomolecular imager. by the R35GM118141- NIGMS-funded investigator Dr. Antoni Barrientos, Ph.D., Professor of Neurology and Biochemistry & Molecular Biology at the University of Miami Miller School of Medicine (UMMSM). ImageQuant™ 800 systems are a new generation of highly sensitive and robust charge-coupled device (CCD) imagers for capture of high-quality images in life science applications. This new range of systems is ideal for chemiluminescence, fluorescence, and colorimetric imaging of a wide variety of samples, including gels, membrane blots, multiwell plates, and petri dishes. Improved optics along with the new SNOW™ (signal-to-noise optimization watch) detection mode allows users to increase both sensitivity and image quality. The system combines an intuitive control software along with ImageQuant CONNECT software, a connection tool which allows access to the imager from remote locations over the network. This instrument will allow us to reduce the volume of X-ray films being used in the lab, and most importantly to increase our versatility, accuracy and experimental rigor.