# High-content microscopy platform for multiplex perturbation/multiplex readout studies

> **NIH NIH R35** · UNIVERSITY OF TX MD ANDERSON CAN CTR · 2024 · $152,999

## Abstract

NOT-GM-24-021 – ABSTRACT – HART
 Highly multiplexed genetic perturbations offer huge potential beyond fitness screens. High throughput
microscopy offers a tractable method for combining complex genetic perturbation with multiparameter functional
assays. Here we propose the purchase of a Nikon Eclipse Ji microscopy system to facilitate complex data
collection to decipher the complexity and redundancy of mammalian signaling pathways. Using our next-
generation CRISPR/Cas12a polygenic knockout system, we combine custom CRISPR arrays targeting signaling
effectors and cell surface “barcodes”, then use immunofluorescence readouts of cell state across dozens of
targets – two to four per well in a 96 well plate -- to understand the dynamics of cell signaling.

## Key facts

- **NIH application ID:** 11098303
- **Project number:** 3R35GM130119-07S1
- **Recipient organization:** UNIVERSITY OF TX MD ANDERSON CAN CTR
- **Principal Investigator:** Traver Hart
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $152,999
- **Award type:** 3
- **Project period:** 2018-08-01 → 2028-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11098303

## Citation

> US National Institutes of Health, RePORTER application 11098303, High-content microscopy platform for multiplex perturbation/multiplex readout studies (3R35GM130119-07S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/11098303. Licensed CC0.

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