Project Summary We are requesting administrative equipment supplemental funds to acquire a preparative HPLC that allows for automated purifications of materials that are difficult to separate by flash column chromatography. This instrument will significantly increase productivity and workflow towards the parent goals of R35GM130387 by enabling rapid purification of crude reactions containing non-volatile products from catalytic reactions on complex molecules. Currently, our research group does not have access to preparative HPLC, let alone preparative systems with the software that allows automated transfer of conditions from analytical to preparative HPLC. A major current focus of researchers studying catalytic reactions, including those of the PI’s laboratory, is to create systems that introduce new functional groups in complex molecules with high selectivity. Multiple projects supported by the PI’s current NIH funding address this general goal and would benefit from this new capability in our group. These include the regioselective borylation, silylation, and alkylation of (sp3)C-H bonds in natural products and complex molecules from medicinal chemistry, the selective oxidation and subsequent derivatization of complex terpenes, and reactions catalyzed by artificial metalloenzymes, including reactions in whole cells. With a method to rapidly purify the products of these reactions, the isomers of the product formed can be identified, and progress on these research goals will be greatly accelerated.