# Mechanisms of Telomere Cohesion - Equipment Supplement

> **NIH NIH R35** · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · 2024 · $40,352

## Abstract

Project Summary Abstract
We request an administrative supplement to grant R35GM149355 for the purchase of an Isothermic Cryogenic
Dry-Vapor Freezer from Custom Biogenic Systems. This is to replace our current Isothermic Cryogenic Dry-
Vapor Freezer which has broken down and is not repairable. The parent grant, R35GM149355, is focused on
mechanisms of telomere cohesion in human cells. The goal of our research is to elucidate the proteins and
mechanisms required for establishment and resolution of sister chromatid cohesion at human telomeres. As
described in the parent grant, the research requires that we generate and store many cell lines. We utilize a
broad range of human cell lines, including primary normal human cell lines and cancer cell lines. We store
multiple aliquots of these cell lines in a cryogenic freezer. For each of these cell lines we use lentivirus to generate
cell lines overexpressing and depleted for the proteins that we proposed to investigate in the grant. We store
multiple aliquots of these cell lines in a cryogenic freezer. We then use these cell lines to measure telomere
function during long term cell growth and aging. Thus, the research in the grant requires that we freeze and store
many cell lines. For this, we require a large, reliable cryogenic storage system. This is essential for the conduct
of the research and for the reproducibility of the studies. Our current system has broken down and it is essential
that we replace it in order to continue the studies proposed in the grant. This request for equipment is completely
within the scope of the parent award.

## Key facts

- **NIH application ID:** 11100494
- **Project number:** 3R35GM149355-02S1
- **Recipient organization:** NEW YORK UNIVERSITY SCHOOL OF MEDICINE
- **Principal Investigator:** SUSAN SMITH
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $40,352
- **Award type:** 3
- **Project period:** 2023-09-11 → 2028-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11100494

## Citation

> US National Institutes of Health, RePORTER application 11100494, Mechanisms of Telomere Cohesion - Equipment Supplement (3R35GM149355-02S1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/11100494. Licensed CC0.

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