# Probing conformational changes by protein surface azidation

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT CHICAGO · 2024 · $155,002

## Abstract

SUMMARY: ADMINISTRATIVE SUPPLEMENT FOR EQUIPMENT (NOT-GM-24-021)
Funds are requested to purchase the Liberty PRIME 2.0 HT12 automated peptide synthesizer. The Adibekian
laboratory currently lacks an automated peptide synthesizer, necessitating the tedious manual synthesis of
shorter peptides using solid-phase synthesis. This equipment is vital for significantly enhancing our capacity to
synthesize longer probe peptides for ProSurA reactions, which are integral to our parent grant R01GM145886.
Systematically performing ProSurA reactions on synthetically pure, precisely defined 20+-mer peptides is
critical to understanding the chemical mechanism of the ProSurA reaction, as well as elucidating the impact of
specific amino acid sequences and secondary and tertiary structures on ProSurA reaction efficiency and rate.
Acquiring the Liberty PRIME automated synthesizer will significantly enhance our experimental capabilities and
accelerate our R01 research program, thereby advancing the mission of the NIGMS and NIH.

## Key facts

- **NIH application ID:** 11100917
- **Project number:** 3R01GM145886-04S1
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT CHICAGO
- **Principal Investigator:** Alexander Adibekian
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $155,002
- **Award type:** 3
- **Project period:** 2022-06-01 → 2026-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11100917

## Citation

> US National Institutes of Health, RePORTER application 11100917, Probing conformational changes by protein surface azidation (3R01GM145886-04S1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/11100917. Licensed CC0.

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