# Synthesis at the Interface of Chemistry and Biology

> **NIH NIH R35** · SCRIPPS RESEARCH INSTITUTE, THE · 2024 · $31,297

## Abstract

Project Summary/Abstract
The unifying theme of our research on synthetic biology is to utilize the principles of Darwinian
evolution to help us understand or expand the fundamental processes of life. As such, the
application of directed evolution to alter, reconstitute, or expand these processes is paramount.
Establishing an automated continuous evolution system comprised of continuous hypermutation
of a gene of interest by a mutagenic T7 replisome, in combination with continuous culturing of its
microbially tractable host E. coli, will greatly accelerate such evolution campaigns in our lab.
Specifically, this will support our research on orthogonal tRNA/RS pairs for genetic code
expansion, as well as the evolution of therapeutic proteases and enzymes.

## Key facts

- **NIH application ID:** 11101049
- **Project number:** 3R35GM145323-03S1
- **Recipient organization:** SCRIPPS RESEARCH INSTITUTE, THE
- **Principal Investigator:** PETER G SCHULTZ
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $31,297
- **Award type:** 3
- **Project period:** 2022-04-01 → 2027-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11101049

## Citation

> US National Institutes of Health, RePORTER application 11101049, Synthesis at the Interface of Chemistry and Biology (3R35GM145323-03S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/11101049. Licensed CC0.

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