# Flow Cytometry Research Core > **NIH NIH P20** · UNIVERSITY OF KANSAS LAWRENCE · 2024 · $75,212 ## Abstract The University of Kansas Flow Cytometry Core E is a Chemical Biology of Infectious Disease (CBID) COBRE Core Laboratory. The Core provides access to flow cytometry and cell sorting instrumentation and expertise to researchers. Services and training are provided for flow cytometry: cell sorting and multi-parametric analysis of individual cells in solution, calculated from their fluorescent or light scattering characteristics. The FCC provides assistance in sample processing, data analysis, instrument training, software support, method and grant assistance, manuscript support, and consulting. The FCC is a 980 N2 BSL-2 facility equipped with a five-laser BD FACSymphony™ S6 Cell Sorter, a four-laser BD FACSAria™ Fusion cell sorter, a five-laser Cytek™ Aurora Spectral Flow Cytometer, and other supplemental assay instrumentation. The Cytek™ Aurora full-spectrum flow cytometry provides users with both tube-based and 96-well plate based spectral cytometry, with five lasers to allow analysis of 30+ colors. The BD FACS instruments allow measurement and sorting of up to six resolved populations of cells simultaneously, based on up to 50 parameters of detection using 18 simultaneous fluorochromes. The facility is equipped to handle BSL-2 samples and perform aseptic and single cell sorting into tubes or 96-well plates. Both FACS cell sorters are enclosed in biosafety cabinets equipped with emergency aerosol management systems (AMS). The core provides instrument training for users who desire to become self-operators of the facility instruments. Core personnel are available for training, assistance with reagent selection or panel development, and project execution. The Flow Cytometry Core is currently staffed by Peter McDonald (Core Director, 100% effort, CBID), and Robin Orozco (Faculty Leader, 10% effort, CBID). These investigators run the sorters, maintain the analyzers and other equipment, and provide expert advice on design and execution of experiments. The core has multiple offline analysis workstations equipped with flow cytometry analysis packages, including a FlowJo™ site license for data analysis software, providing the components and advanced software for post-acquisition data analysis and graphics generation. The core stores and protects user data on a research file storage network, protected by a RAID system. Stored data is accessible within the KU network or remotely via the university's virtual private network. The core also features proximity key card access to the core for secure 24-hour access. The core is currently utilized by eight academic departments, with over 50 investigators with 38% who are CBID affiliated. The FC core will equip CBID researchers with tools directly applicable to infectious disease research, such as identifying and characterizing infectious agents such as bacteria and parasites, quantification and sorting of cells infected with microbial pathogens, and assessing chemical probe efficacy against ... ## Key facts - **NIH application ID:** 11112149 - **Project number:** 5P20GM113117-09 - **Recipient organization:** UNIVERSITY OF KANSAS LAWRENCE - **Principal Investigator:** Robin C. Orozco - **Activity code:** P20 (R01, R21, SBIR, etc.) - **Funding institute:** NIH - **Fiscal year:** 2024 - **Award amount:** $75,212 - **Award type:** 5 - **Project period:** 2024-06-01 → 2026-05-31 ## Primary source NIH RePORTER: https://reporter.nih.gov/project-details/11112149 ## Citation > US National Institutes of Health, RePORTER application 11112149, Flow Cytometry Research Core (5P20GM113117-09). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/11112149. Licensed CC0. --- *[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*