# Advanced Single Cell Technology Innovation Core

> **NIH NIH U54** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2024 · $259,748

## Abstract

PROJECT SUMMARY
The proposed Cancer Systems Therapeutics (CaST) Center focuses on understanding and targeting molecularly 
distinct yet co-existing cell states and transitions that establish and maintain tumors and tumor 
microenvironments at a systems level. These goals demand analytical methods with single-cell resolution. The 
Advanced Single Cell Technology Innovation Core (ASCeTIC) will provide state-of-the-art technology for single-cell genomic analysis, multiplexed single-cell molecular perturbation, and in situ validation to CaST investigators. 
We will offer a suite of tools including multi-omic methods for joint single-cell RNA/ATAC-seq, single-cell 
imaging/RNA-seq (SCOPE-seq), and single-cell CRISPRi/RNA-seq (CROP-seq). Recognizing the importance 
of spatial profiling for both in situ validation and studies of cell-cell interactions in the tumor microenvironment, 
we will also offer immunohistochemical analysis, RNA-FISH, and cyclic immunofluorescence (cycIF), which can 
simultaneously quantify tens of protein epitopes in individual cells in a single tissue section.

## Key facts

- **NIH application ID:** 11171868
- **Project number:** 5U54CA274506-02
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Peter Alan Sims
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2024
- **Award amount:** $259,748
- **Award type:** 5
- **Project period:** 2023-09-19 → 2028-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11171868

## Citation

> US National Institutes of Health, RePORTER application 11171868, Advanced Single Cell Technology Innovation Core (5U54CA274506-02). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/11171868. Licensed CC0.

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