Differential redox regulation of vein and arterial smooth muscle cells in AVF stenosis

NIH RePORTER · NIH · R56 · $200,000 · view on reporter.nih.gov ↗

Abstract

(PLEASE KEEP IN WORD, DO NOT PDF) Kidney failure impacts many, with 750,000 cases annually in the US and 2 million globally. Treatment options include kidney transplant or dialysis. Hemodialysis requires vascular access, often through arteriovenous fistulae (AVF) for improved long-term survival and reduced infection risk when compared to grafts and catheters. Nevertheless, AVFs frequently mature inadequately, exceeding 50% within 6 months, leading to interventions and complications due to neointimal hyperplasia (NH) and inadequate outward remodeling. Vascular injury results in the phenotypic modulation of vascular smooth muscle cells (VSMCs), pathognomonic of NH. Oxidative stress emerges as a pivotal contributor to these downstream processes. Consequently, interventions aimed at mitigating oxidative stress through antioxidant therapies have the potential to enhance AVF maturation, especially since patients with kidney failure have elevated levels of oxidative stress. Our hypothesis posits that Nrf-2 activation in VSMCs and in an in vivo mouse AVF stenosis model, particularly in uremia, can inhibit VSMC phenotypic changes, and reduce NH while promoting outward remodeling, thus improving AVF maturation. We will investigate this through: Studying the effect of Nrf2 activation on the phenotype of VSMC Assessing a new local therapeutic approach that activates Nrf2 in a mouse AVF stenosis model. Successful completion of this proposal holds the potential to alleviate clinical morbidity associated with AVF failure, which remains our foremost objective, driven by the pressing needs of our patients.

Key facts

NIH application ID
11174188
Project number
1R56DK140967-01
Recipient
UNIV OF NORTH CAROLINA CHAPEL HILL
Principal Investigator
Edward M Bahnson
Activity code
R56
Funding institute
NIH
Fiscal year
2024
Award amount
$200,000
Award type
1
Project period
2024-09-17 → 2025-09-16