# The role of microRNA-210 in regulating oxidative stress in patients with peripheral artery disease

> **NIH NIH R01** · UNIVERSITY OF WEST FLORIDA · 2023 · $521,511

## Abstract

Abstract
Peripheral artery disease (PAD) affects 8.5 million of Americans over 40 years of age. mircroRNAs (miRs) are
endogenous 21∼25 nucleotides noncoding RNA, that can regulate posttranscriptional gene expression. The
most common mechanism of action of miRs is by binding to the 3' un-translated region of a target mRNA and
thereby reducing mRNA expression or protein translation. Circulating miRNAs, represent potential biomarkers
for the diagnosis and prognosis of PAD and a start point for individualized treatment. Recent evidence from our
first R01 suggests that mircroRNA-210 (miR-210) expression in the skeletal muscle of PAD patients (intermittent
claudication and critical limb ischemia) is an important biomarker that targets the iron-sulfur cluster scaffold
homolog and cytochrome c oxidase assembly protein genes leading to decreased mitochondrial respiration. In
this R01 renewal we want to go a step further and investigate all the targets of miR-210 using an omics approach.
We are bringing together an excellent team of vascular surgeons, biomedical researchers and omics experts to
investigate how miR-210 and its downstream targets affect the skeletal muscle and the vasculature of PAD and
non-PAD controls. To that end, Aim #1, will identify downstream targets of miR-210 using an omics approach in
PAD and non-PAD controls. Aim #2, will determine the effect of revascularization operations in decreasing miR-
210 expression and associate these changes with walking function and quality of life parameters. Aim#3, will
determine health disparities in Hispanic origin PAD patients. Addressing these aims, will provide for the first time,
a complete analysis of all the changes in skeletal muscle and vasculature of PAD patients that undergoing a
revascularization operation using the omics approach.

## Key facts

- **NIH application ID:** 11220628
- **Project number:** 7R01AG064420-07
- **Recipient organization:** UNIVERSITY OF WEST FLORIDA
- **Principal Investigator:** Panagiotis Koutakis
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2023
- **Award amount:** $521,511
- **Award type:** 7
- **Project period:** 2019-08-15 → 2026-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11220628

## Citation

> US National Institutes of Health, RePORTER application 11220628, The role of microRNA-210 in regulating oxidative stress in patients with peripheral artery disease (7R01AG064420-07). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/11220628. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*