# Genetically Engineered Mouse Models (GEMM)

> **NIH CA P30** · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · 2026 · $296,449

## Abstract

PROJECT SUMMARY/ABSTRACT – GENETICALLY ENGINEERED MOUSE MODELS SHARED
RESOURCE
The Genetically Engineered Mouse Models Shared Resource (GEMMSR), formerly the Transgenic Gene
Knockout Shared Resource, provides St. Jude Comprehensive Cancer Center (SJCCC) members with
convenient and economic access to generating genetically engineered mouse models (GEMMs). This includes
the production of transgenic, knock-out, conditional knock-out, deletion, and knock-in mouse lines. These models
are critical tools for advancing science at the SJCCC by elucidating the role of genetic changes in the initiation
and progression of cancer and deciphering the mechanisms through which therapeutic targets operate in
anticancer treatment. The GEMMSR is directed by Youngwook Ahn, PhD, who has over 18 years of transgenic
and GEMM creation experience and is supported by three full-time, highly experienced staff members. The
GEMMSR provides a range of services, including consultation, classic transgenics, CRISPR editing via
pronuclear injection, CRISPR editing via electroporation, and extended genotyping. The CRISPR editing with
electroporation of zygotes and extended genotyping services were newly introduced during the project period.
With this additional method for creating mouse lines, the GEMMSR increased the live birth rates of all GEMMs
from ~6% to ~25% and reduced the number of zygotes required. During the project period, several additional
changes were made. The GEMMSR retired the mouse embryonic stem cell arm of the facility due to decreased
demand, allowing for a greater focus on CRISPR-based animal model creation. Additionally, the GEMMSR
decided to stop maintaining an in-house C57BL/6 donor colony, opting instead to purchase these mice from an
external vendor. This change reduced the overall number of animals and cages maintained and contributed to
an increase in live birth rates. Due to these changes and increases in efficiency, GEMMSR staffing was reduced
by two staff members, whil

## Key facts

- **NIH application ID:** 11265065
- **Project number:** 2P30CA021765-47
- **Recipient organization:** ST. JUDE CHILDREN'S RESEARCH HOSPITAL
- **Principal Investigator:** Youngwook  Ahn
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** CA
- **Fiscal year:** 2026
- **Award amount:** $296,449
- **Award type:** 2
- **Project period:** 1997-04-01T00:00:00 → 2031-03-31T00:00:00

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11265065

## Citation

> US National Institutes of Health, RePORTER application 11265065, Genetically Engineered Mouse Models (GEMM) (2P30CA021765-47). Retrieved via AI Analytics 2026-05-20 from https://api.ai-analytics.org/grant/nih/11265065. Licensed CC0.

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