# GPCR signaling during embryonic organ formation

> **NIH GM R01** · JOHNS HOPKINS UNIVERSITY · 2026 · $408,780

## Abstract

G-protein coupled receptors (GPCRs) are critical for almost every aspect of animal life. These
proteins are embedded in the cell membrane and allow us to sense and respond to light, smells,
and taste. GPCRs also control responses in both our central and autonomic nervous systems,
and they regulate both inflammation and immunity. GPCRs control cell migration for normal
development and during cancer metastasis. Indeed, approximately 34% of FDA-approved drugs
target GPCRs. Nonetheless, despite decades of study, many GPCRs have no known function,
their ligands remain unidentified, and the pathways through which they elicit distinct cellular
responses remain mostly uncharacterized. Here, we propose to take the first steps toward
understanding the roles of GPCRs during development in the experimental system of the
Drosophila embryo, which has numerous advantages in terms of visual accessibility, an
extensive armamentarium of genetic tools, and relatively low cost.
We begin with an analysis of the Drosophila GPCR Tre1, which has been implicated in germ
cell (GC) navigation and survival, extravasation of immune cell to sites of injury, and polarization
of neuroblasts. We have recently reported that non-canonical Hedgehog signaling works
through the Tre1 receptor to control GC navigation, resolving a long-standing conflict regarding
the role of Hh in this process and revealing a novel pathway downstream of Tre1 activation. In
the first aim, we uncover the molecular and cellular mechanisms through which each step of this
pathway is mediated – from receptor binding to actin polymerization. We ask if and how other
genes that affect GC migration work through this pathway to repel GCs (in the case of the
Wunen lipid phosphate phosphatases) or attract GCs (in the case of HMGCoA reductase). Tre1
is also expressed in the forming salivary gland (SG), a tissue that, unlike GCs, migrates as a
fully polarized epithelial collective. We ask if Hh signaling and Tre1 also function in the SG

## Key facts

- **NIH application ID:** 11291795
- **Project number:** 5R01GM145873-04
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **Principal Investigator:** Deborah J Andrew
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** GM
- **Fiscal year:** 2026
- **Award amount:** $408,780
- **Award type:** 5
- **Project period:** 2023-04-01T00:00:00 → 2027-02-28T00:00:00

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11291795

## Citation

> US National Institutes of Health, RePORTER application 11291795, GPCR signaling during embryonic organ formation (5R01GM145873-04). Retrieved via AI Analytics 2026-05-20 from https://api.ai-analytics.org/grant/nih/11291795. Licensed CC0.

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