# Deciphering a tissue specific role for Arhgap29 during palatogenesis

> **NIH DE F31** · UNIVERSITY OF IOWA · 2026 · $37,664

## Abstract

PROJECT SUMMARY
 Orofacial clefts (OFCs), specifically cleft lip with or without cleft palate, are among the most common
class of birth defects and they contribute to a significant health and financial burden. The development of OFCs
is influenced by complex interactions between genetic and environmental factors. Developing a comprehensive
understanding of the cellular pathways and molecular regulators of palatogenesis will be essential to enhancing
treatment options and disease intervention. My overall objective of the proposed project is to identify molecular
pathways that modulate palatal development. Genetic studies in the human identified over 50 loci associated
with OFC. However, mutations in very few of these genes can cause isolated OFC with high penetrance, making
them well-suited for mechanistic studies and better candidates for clinical interventions. One such gene is Rho
GTPase activating protein 29 (ARHGAP29). ARHGAP29 contributes to cyclic regulation of the small GTPase
RhoA, inactivating it. To explore the role of ARHGAP29 during craniofacial development, Arhgap29 was
previously deleted in the mouse. Although Arhgap29 knockout embryos were found to die around embryonic day
e8.5, i.e., before craniofacial structures develop, heterozygous loss-of-function embryos were viable and
displayed intraoral adhesions, a phenotype associated with OFCs. In the craniofacial region, ARHGAP29 is
expressed in cell lineages derived from both the ectoderm (periderm and epithelial cell layers) and the neural
crest (mesenchymal cells). To tease apart the contributions of ARHGAP29 to each cell lineage during
palatogenesis, I initiated a tissue-specific knockout strategy using the Cre-Lox system. My preliminary results
from these animals show that the loss of ARHGAP29 in either ectoderm- or neural crest-derived lineages results
in a delay in palatogenesis (apparent at e14.5). However, only the loss of ARHGAP29 in ectoderm-derived cells
results in a cleft palate at e18.5. Th

## Key facts

- **NIH application ID:** 11308250
- **Project number:** 5F31DE033222-03
- **Recipient organization:** UNIVERSITY OF IOWA
- **Principal Investigator:** Emily  Adelizzi
- **Activity code:** F31 (R01, R21, SBIR, etc.)
- **Funding institute:** DE
- **Fiscal year:** 2026
- **Award amount:** $37,664
- **Award type:** 5
- **Project period:** 2024-03-01T00:00:00 → 2027-02-28T00:00:00

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11308250

## Citation

> US National Institutes of Health, RePORTER application 11308250, Deciphering a tissue specific role for Arhgap29 during palatogenesis (5F31DE033222-03). Retrieved via AI Analytics 2026-05-20 from https://api.ai-analytics.org/grant/nih/11308250. Licensed CC0.

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