# De novo design of photoacid-binding proteins to study proton dynamics in biological systems

> **NIH GM F32** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2026 · $79,348

## Abstract

Project Summary/Abstract
The goal of this proposal is to elucidate biological mechanisms for proton transfer by designing function from
scratch. The coupled movement of protons and electrons is crucial to biological energy transduction and central
to life. While electron transfer (ET) has been extensively studied, less is known about the corresponding proton
transfer (PT) due to lack of easily observable experimental readouts. Computational protein design enables us
to study these phenomena in a ground-up manner where a protein scaffold can be designed from first principles
to mimic biological function in isolated and experimentally tractable ways. This proposal centers on the binding
of abiological photoacid cofactors that would give distinct spectroscopic readouts for PT as a function of distance.
The electron-deficient metal porphyrin photoacid cofactors used in this proposal are characterized by dramatic
acidification upon photoexcitation and distinct spectroscopic changes upon deprotonation. These cofactor
properties combined with our lab’s history of success in the design of porphyrin-binding proteins make them ideal
for use in this proposal. Using computational tools recently developed in the DeGrado lab (vdMs and COMBS),
the cofactor will be positioned within a designer protein scaffold H-bonded to a proton-accepting residue. This
will enable the spectroscopic study of proton on-off rates upon irradiation and subsequent deprotonation of the
cofactor. These ligand-binding proteins will be experimentally characterized through X-ray crystallography and
NMR experiments to validate the proposed structure and binding mode. Ultrafast absorbance spectroscopy
experiments will be carried out by our long-term collaborators in the Therien lab at Duke University. Following
characterization, the proton-accepting residue will be iteratively moved down the protein scaffold with a designed
“water-wire” in its wake to allow spectroscopic observation of the proton movement ove

## Key facts

- **NIH application ID:** 11309090
- **Project number:** 5F32GM154484-03
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Ian  Bakanas
- **Activity code:** F32 (R01, R21, SBIR, etc.)
- **Funding institute:** GM
- **Fiscal year:** 2026
- **Award amount:** $79,348
- **Award type:** 5
- **Project period:** 2024-04-01T00:00:00 → 2027-03-31T00:00:00

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11309090

## Citation

> US National Institutes of Health, RePORTER application 11309090, De novo design of photoacid-binding proteins to study proton dynamics in biological systems (5F32GM154484-03). Retrieved via AI Analytics 2026-07-13 from https://api.ai-analytics.org/grant/nih/11309090. Licensed CC0.

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