# Genetic and Proteomic Approaches to Reveal Bacterial Vulnerabilities to Phage Predation

> **NIH AI R01** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2026 · $667,537

## Abstract

PROJECT SUMMARY
We are entering a post antibiotic world where understanding the mechanism of an antibacterial strategy is not
sufficient to ensure an effective therapy. We must also consider the mechanisms of resistance and address them
during the design process. We will use genetics and proteomics to discover how bacteria combat bacteriophage
(phage) lysis. Driving this goal is the desire to combat phage resistance mechanisms so as to make bacteria
more susceptible to phage predation. We first tackle the problem by employing state of the art genetic methods
to interrogate the role of essential genes in limiting phage replication and bacterial lysis. Using CRISPR
transcriptional interference (CRISPRi), we will conduct the first studies in the human pathogen Pseudomonas
aeruginosa to determine whether partial knockdown of essential genes can positively impact phage replication.
We hypothesize that inhibition of certain essential genes will not only limit bacterial fitness, but also has the
potential to enhance the success of any phage-host pairing, regardless of whether the a priori state is one of
phage resistance or sensitivity. Put another way, even phages that already replicate in a given host can do better.
We will additionally harness the ease of CRISPRi screening to identify non-essential genes that limit phage
replication in strain-dependent manners, more akin to canonical hypervariable immune systems (e.g. CRISPR).
To further our understanding of the physical underpinnings of phage resistance, we will create a physical map
of phage-host protein-protein interactions using whole cell fractionation proteomics. This is particularly critical as
many phage proteins are of unknown function and is in line with our goal of identifying essential protein
complexes interacting with phage factors. We will validate the importance of factors identified from genetic and
proteomic assays with phage replication assays during single gene knockdown to assess generalities of
phe

## Key facts

- **NIH application ID:** 11323156
- **Project number:** 5R01AI167412-05
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Joseph  Bondy-Denomy
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** AI
- **Fiscal year:** 2026
- **Award amount:** $667,537
- **Award type:** 5
- **Project period:** 2022-05-20T00:00:00 → 2027-04-30T00:00:00

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11323156

## Citation

> US National Institutes of Health, RePORTER application 11323156, Genetic and Proteomic Approaches to Reveal Bacterial Vulnerabilities to Phage Predation (5R01AI167412-05). Retrieved via AI Analytics 2026-07-13 from https://api.ai-analytics.org/grant/nih/11323156. Licensed CC0.

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