# Multi-Omics Analysis of Broadly Neutralizing Antibodies and Therapeutic Vaccination

> **NIH AI P01** · BETH ISRAEL DEACONESS MEDICAL CENTER · 2026 · $1,500,997

## Abstract

SUMMARY
The goal of this P01 program is to define mechanisms of efficacy of broadly neutralizing antibodies (bNAbs) and
therapeutic vaccines for HIV-1 in a highly collaborative and multifaceted research program. Our overall
hypothesis is that immunologic strategies for HIV-1 cure delay viral rebound both by targeting the viral
reservoir and by increasing host antiviral immunity. We will evaluate mechanisms of therapeutic efficacy in
both humans and nonhuman primates (NHPs) using coordinated virologic, immunologic, and multi-omic
approaches with the goal of developing improved next generation HIV-1 cure strategies. We will apply cutting-
edge, high-throughput, multi-omic profiling platforms and integrate these data sets to generate a comprehensive
tissue landscape and regulatory network of the viral reservoir and host immune responses.
This program builds on our existing studies over the past several years in both humans and NHPs evaluating
the ability of bNAbs and therapeutic vaccines to delay viral rebound following discontinuation of antiretroviral
therapy (ART). We will first utilize existing samples from these studies to generate hypotheses regarding
correlates of delayed viral rebound. We will then perform new interventional studies in NHPs with spatial multi-
omic analyses in lymph nodes and gastrointestinal mucosal tissues to test hypotheses in order to define
mechanisms of reservoir targeting by bNAbs and therapeutic vaccines.
The significance of this program is the potential to define the biologic pathways that lead to delayed viral rebound
following ART discontinuation. An improved understanding of partially effective bNAbs and therapeutic vaccines
in humans and NHPs will lead to basic research advances that will allow the development of improved next
generation HIV-1 cure approaches. To accomplish the goals of this P01 program, we propose the following
Projects and Cores:
Project 1. Multi-Omics Correlates of Broadly Neutralizing Antibody Efficacy
Project 

## Key facts

- **NIH application ID:** 11330320
- **Project number:** 5P01AI177687-04
- **Recipient organization:** BETH ISRAEL DEACONESS MEDICAL CENTER
- **Principal Investigator:** Dan H. Barouch
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** AI
- **Fiscal year:** 2026
- **Award amount:** $1,500,997
- **Award type:** 5
- **Project period:** 2023-07-11T00:00:00 → 2028-04-30T00:00:00

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11330320

## Citation

> US National Institutes of Health, RePORTER application 11330320, Multi-Omics Analysis of Broadly Neutralizing Antibodies and Therapeutic Vaccination (5P01AI177687-04). Retrieved via AI Analytics 2026-06-26 from https://api.ai-analytics.org/grant/nih/11330320. Licensed CC0.

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