# Core-001

> **NIH AI P01** · UNIVERSITY OF CHICAGO · 2026 · $755,481

## Abstract

Project Summary/Abstract 
The purpose of the Animal and Microsurgery Core (Core B) is to establish a focused facility that provides 
investigators in each of the two projects with sufficient breeding of animals with specified genotypes, and with 
standardized cardiac or skin transplantation and tolerance induction procedures for the proposed experiments. 
Core B will provide a key service role that will enable the Principal Investigators and their staff to focus on 
experimental and scientific efforts. Centralized breeding and screening of the progeny will ensure that animals 
of required genotypes are available for the proposed experiments. Transplantation performed by a centralized 
source will standardize the procedures, minimize surgically imposed disparities in the grafts used by the two 
projects and allow some long-term acceptors generated for Project 1 (addressing vulnerabilities to the 
maintenance of transplantation tolerance) to be used for Project 2 (confronting the barrier to stable 
transplantation tolerance posed by memory T cells) to minimize experimental costs. In addition, the purchase of 
supplies, such as sutures, anesthetics and immunosuppressants will be done in bulk for both projects to reduce 
cost. The specific tasks of Core B will be to: a) breed and genotype animals to be used for both projects; b) 
transplant heart allografts as directed by the project leaders and their personnel; c) prepare and inject donor 
splenocytes and administer indicated immunosuppressive therapies; d) monitor the beating of the transplanted 
hearts, the health of the transplant recipients, and record times of rejection; e) sacrifice transplanted animals as 
directed by the Principal Investigators and their personnel, and collect organs for histology, as well as to distribute 
to other project personnel for phenotypic and functional analyses of immune cells.

## Key facts

- **NIH application ID:** 11528761
- **Project number:** 5P01AI097113-14
- **Recipient organization:** UNIVERSITY OF CHICAGO
- **Principal Investigator:** Maria-Luisa  Alegre; Anita S Chong
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** AI
- **Fiscal year:** 2026
- **Award amount:** $755,481
- **Award type:** 5
- **Project period:** 2012-07-17T00:00:00 → 2028-04-30T00:00:00

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/11528761

## Citation

> US National Institutes of Health, RePORTER application 11528761, Core-001 (5P01AI097113-14). Retrieved via AI Analytics 2026-05-20 from https://api.ai-analytics.org/grant/nih/11528761. Licensed CC0.

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