# Priming of vascular tube morphogenesis: Novel role for VEGF and downstreamRhoA activation

> **NIH NIH R01** · UNIVERSITY OF SOUTH FLORIDA · 2020 · $389,375

## Abstract

In this new collaborative proposal, we investigate our novel findings regarding the ability of VEGF to act as an
upstream vascular morphogenic primer through activation of the small GTPase, RhoA, during mouse vascular
development and in human ECs in vitro by examining blood vessel assembly. The Cleaver lab has shown that
VEGFR2 or RhoA inactivation, at or before the appearance of angioblasts in mice, leads to complete loss of
EC tubulogenesis, while disruption of RhoA later after EC tube formation leads to marked enlargement of EC
tubes, suggestive of temporally distinct roles (tubulogenesis early, restraint of vessel enlargement later). In
addition, new work from the Cleaver lab reveals that inactivation of Cdc42 at early or later time points during
vascular development leads to marked defects in EC tubulogenesis. The Davis lab observes the same in vivo
phenotypes using in vitro EC tubulogenesis assays. Recently, the Davis lab has defined growth factor
requirements for EC tubulogenesis and EC-pericyte tube co-assembly in 3D matrices showing that SCF, IL-3,
SDF-1α, FGF-2, and insulin (GFs) are necessary for these processes under serum-free defined conditions.
Importantly, VEGF addition is not required for this defined GF-driven morphogenic process, yet it has profound
effects in vivo, just like the influence of RhoA. Our collaborative work has led to a fundamental and paradigm-
shifting observation demonstrating that VEGF acts as an upstream primer through RhoA activation to prepare
ECs/angioblasts for downstream vascular morphogenic events. In fact, VEGF treatment of ECs specifically
primes their responses to these pro-tubulogenic GFs; which directly stimulate an increase in EC tip cells, EC-
lined tubes and pericyte recruitment to EC tubes. To elucidate VEGF priming signals, we show that VEGF
promotes RhoA activation leading to formation of actin stress fibers with increased focal adhesions and
tyrosine phosphorylation of FAK and paxillin, and also activates protein kinase D (PKD) and Hsp27. siRNA
suppression of VEGFR2, RhoA, and PKD2 markedly interferes with VEGF-induced priming. Together, these
new insights define a novel step during blood vessel formation, EC priming, and provide a molecular road map
to dissect how VEGF acts as a primer through RhoA activation to control blood vessel assembly.
 We propose three specific aims to further investigate these novel insights into the fundamental
process of VEGF-induced and RhoA-dependent EC priming in vitro and in vivo and they are;
Aim1: To test VEGF-dependent EC signaling and RhoA activation as central regulators of priming, in vitro and
in vivo.
Aim2: To identify and characterize key RhoGEFs which activate RhoA in conjunction with VEGFR2-dependent
signaling, in order to prime ECs for subsequent tube morphogenic events.
Aim3: To investigate fundamental EC mechanisms that suppress VEGF priming and RhoA activation,
including the role of Rasip1 and Arhgap29, which are inhibitors of RhoA activation.

## Key facts

- **NIH application ID:** 9731289
- **Project number:** 5R01HL136139-04
- **Recipient organization:** UNIVERSITY OF SOUTH FLORIDA
- **Principal Investigator:** Ondine B Cleaver
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $389,375
- **Award type:** 5
- **Project period:** 2017-09-01 → 2024-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9731289

## Citation

> US National Institutes of Health, RePORTER application 9731289, Priming of vascular tube morphogenesis: Novel role for VEGF and downstreamRhoA activation (5R01HL136139-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9731289. Licensed CC0.

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