# Chd3 Conditional Knockout Mice:  A New Tool for Studies of Lymphocyte Epigenetics

> **NIH NIH R03** · NATIONAL JEWISH HEALTH · 2020 · $82,000

## Abstract

PROJECT SUMMARY
The generation of B and T lymphocytes follows their progression from uncommitted
progenitors through a series of defined stages of development. At each stage, cells
express a distinct set of genes, or transcriptome. These patterns of gene expression are
controlled, in part, by tissue- and stage-specific epigenetic factors that limit the
accessibility of chromatin. We have demonstrated that Nucleosome Remodeling and
Deacetylase (NuRD) complexes, which include the Chromodomain Helicase and DNA
binding 3 (CHD3; also known as Mi-2α) or the related protein CHD4 (Mi-2β) proteins, are
gatekeepers of gene transcription. CHD4 has been studied extensively in B and T cells,
while very little is known concerning its closely related paralog CHD3. The literature
suggests that normal lymphocyte development likely requires both CHD proteins due to
their distinct functions, but this has not been proven in an in vivo model system. Here,
we will generate gene-targeted mice for the conditional knockout of Chd3 genes in
lymphocytes of mice. These mice are currently unavailable commercially and from other
research laboratories. Thus, we will generate a valuable resource that can be shared
with other investigators with interests in lymphocyte development, epigenetics, and
specific functions of NuRD complexes.

## Key facts

- **NIH application ID:** 9823849
- **Project number:** 5R03AI139822-02
- **Recipient organization:** NATIONAL JEWISH HEALTH
- **Principal Investigator:** James R. Hagman
- **Activity code:** R03 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $82,000
- **Award type:** 5
- **Project period:** 2018-11-15 → 2021-10-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9823849

## Citation

> US National Institutes of Health, RePORTER application 9823849, Chd3 Conditional Knockout Mice:  A New Tool for Studies of Lymphocyte Epigenetics (5R03AI139822-02). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9823849. Licensed CC0.

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