# The human temporal bone laboratory resource for basic and applied inner ear sciences

> **NIH NIH U24** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 2020 · $627,495

## Abstract

ABSTRACT
In the present proposal we will preserve, process and make available human ear tissue specimens for inner
ear basic science researchers such that they may be able to compare with their animal models. The use of
celloidin and microdissected human temporal bone tissue for immunohistochemical and quantitative
techniques will be instrumental in driving basic animal inner ear research forward towards understanding of
human ear pathology and has high potential for the development of effective drug therapies for restoring
auditory and vestibular function. In Specific Aim 1) we will improve access for the auditory and vestibular
research community to human ear tissues from temporal bone specimens. We will improve and promote timely
procurement, preparing and processing of new specimens, and appropriate re-processing of archived
specimens. We will distribute a limited number of well-prepared laboratory tissues to funded researchers who
lack adequate access to facilities for human temporal bone preparation. We will also exploit use of the
Registry for information about currently archived tissues and their access by the community, and promote
collaborations between researchers seeking to validate animal models for human relevance. Specific Aim 2)
We will optimize tissue preparation techniques. We will include molecular and cellular marking and
visualization techniques that allow direct comparisons of fresh and archived human tissues with those from
animal models. We will disseminate protocols for procurement, preservation and preparation of human ear
tissues from temporal bones. By doing this we will encourage animal researchers to include human tissues into
their analyses. Specific Aim 3) We will use high resolution MRI to evaluate the fluid-filled inner ear structures,
and ex vivo MRI to evaluate the inner ear structures post-mortem and make comparisons with celloidin-
embedded histopathology. We will exploit new technologies and methods to image and visualize human
middle and inner ears in situ with the aim of visualizing soft cellular tissues, membranes and fluids, as well as
hard bony tissues. Specific Aim 4) We will provide technical instruction with hands-on training will be available
for investigators, technicians otolaryngology residents, medical school and graduate students interested in
inner ear biology in the temporal bone processing techniques and skills needed for processing human ear
tissues from temporal bones. The materials generate in this proposal will be mutually beneficial for researchers
in basic scientific studies of the inner ear, as well as to temporal bone scientists wishing to apply high levels of
molecular and cellular techniques to the human temporal bone. These collaborative efforts hold promise to
rapidly accelerate the science of temporal bone processing and the understanding of human otopathologies.

## Key facts

- **NIH application ID:** 9824459
- **Project number:** 5U24DC015910-04
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **Principal Investigator:** Akira Ishiyama
- **Activity code:** U24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $627,495
- **Award type:** 5
- **Project period:** 2016-12-01 → 2021-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9824459

## Citation

> US National Institutes of Health, RePORTER application 9824459, The human temporal bone laboratory resource for basic and applied inner ear sciences (5U24DC015910-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9824459. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*