# Flow Cytometry

> **NIH NIH P30** · UNIV OF NORTH CAROLINA CHAPEL HILL · 2020 · $169,218

## Abstract

ABSTRACT-Flow Cytometry (FLOW) Shared Resource 
The Flow Cytometry Shared Resource (FLOW SR) is a basic shared resource in the basic group that provides 
flow cytometry research support for Cancer Center members. The overall mission of the SR is to provide 
technical, educational assistance for individuals performing flow cytometry and to assist investigators in data 
interpretation and the planning of future experiments that involve flow cytometry. The SR provides multi-color 
flow cytometry analysis, high speed sorting (including biohazard sorting), education, consultation, application 
development, and data analysis. Since the 2010 review, use of the shared resource has increased 63%. To 
accommodate and permit this increase the SR has increased both hardware and staff. We added two 
analytical cytometers: a Stratedigm S1000EX for microparticle analysis, a 4-laser BD LSRFortessa, and two 
cell sorters: a BD FACSAria II SORP in BSL-2 containment and a FACSAria III for investigator operation. The 
FACSAria II, which is configured for BSL-2 sorting has been placed in a specially renovated BSL-2 space with 
enhanced precautions. We also upgraded the Beckman MoFlo XDP with two additional lasers. Education 
initiatives have expanded considerably, including user-operated cell sorting on the FACSAria III. The Specific 
Aims focus on providing access and training on existing equipment, integrating new equipment, providing 
expert research staff and provided that continuing education to users on new experimental methods. We 
added a highly skilled Technology Manager position to accommodate the increased use and to provide 
additional services including expertise for the incoming Amnis ImageStreamX imaging flow cytometer. Center 
member usage accounted for 54% of the total SR usage and resulted in publications in journals such as JCI, 
JPET, and Blood. For 2015, the FLOW SR Facility requests $122,656 for personnel and maintenance 
expenses. CCSG funds are projected to be 19% of operating costs.

## Key facts

- **NIH application ID:** 9834848
- **Project number:** 5P30CA016086-44
- **Recipient organization:** UNIV OF NORTH CAROLINA CHAPEL HILL
- **Principal Investigator:** NANCY C. FISHER
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $169,218
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9834848

## Citation

> US National Institutes of Health, RePORTER application 9834848, Flow Cytometry (5P30CA016086-44). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9834848. Licensed CC0.

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