# Proteomics

> **NIH NIH P30** · UNIV OF NORTH CAROLINA CHAPEL HILL · 2020 · $195,939

## Abstract

ABSTRACT – Proteomics (PROT) Shared Resource 
The UNC Proteomics (PROT) Shared Resource (SR) provides state-of-the-art mass spectrometry-based 
qualitative and quantitative protein analyses for LCCC members. The PROT SR offers a variety of services to 
cancer researchers including: guidance in experimental design; protein and peptide identification; relative and 
absolute protein quantitation; analysis of protein modifications; activity-based proteomics; instrument training; 
and proteomic informatics. In addition to these basic proteomic services there are four cancer proteomic 
research themes, each involving active collaborations with LCCC investigators, which have been developed 
since the last renewal. These themes provide state-of-the art proteomic research technologies to members of 
the Cancer Center and include: i) chemical proteomics methods to interrogate the dynamic activation state of 
the cancer kinome; ii) quantifying both the proteome and phosphoproteome of patient tumors as part of the 
NCI CPTAC initiative; iii) mass spectrometry and computational methods for defining protein-protein interaction 
networks in cancer, including quantitative measurement of network dynamics; and iv) absolute quantification of 
proteins in complex protein mixtures by selective reaction monitoring (SRMs). 
The PROT SR is led by Lee Graves (MT), Professor of Pharmacology and director, and David Smalley, 
Facility Director. Three additional faculty are directly involved with the efforts of the PROT SR: Gary Johnson 
(MT), Kenan Distinguished Professor and Chair of the Department of Pharmacology and co-director of the 
Molecular Therapeutics Program, Ben Major (CCB), Associate Professor of Cell Biology & Physiology, and 
Xian Chen (IM), Professor of Biochemistry & Biophysics and technology director of the PROT SR. Together 
they provide strong synergistic leadership in mass spectrometry based proteomics with a proven record of 
productive collaboration with investigators in the Cancer Center.

## Key facts

- **NIH application ID:** 9834850
- **Project number:** 5P30CA016086-44
- **Recipient organization:** UNIV OF NORTH CAROLINA CHAPEL HILL
- **Principal Investigator:** Lee M Graves
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $195,939
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9834850

## Citation

> US National Institutes of Health, RePORTER application 9834850, Proteomics (5P30CA016086-44). Retrieved via AI Analytics 2026-05-28 from https://api.ai-analytics.org/grant/nih/9834850. Licensed CC0.

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