# Transgenic and Chimeric Mouse Facility

> **NIH NIH P30** · UNIVERSITY OF PENNSYLVANIA · 2020 · $196,501

## Abstract

Project Summary
The Transgenic and Chimeric Mouse Facility has been continuously funded by the NCI CCSG since 1989. Drs.
Stephen Liebhaber and Nancy Cooke, Professors of Genetics and Medicine, have provided leadership as Co-
Directors to this Shared Resource since 1993. Both individuals are experienced investigators with considerable
expertise in transgenic and chimeric mouse generation and analysis, and mammalian gene targeting. An
experienced technical team led by Dr. Jean Richa provides expertise in a range of transgenic technologies,
enabling the facility to regularly introduce new and improved services. Among the services provided by the
Transgenic and Chimeric Mouse Facility are: generation of transgenic mice via microinjections of DNA into
fertilized oocyte; generation of chimeric mice via injection of embryonic stem cells into blastocysts; direct
genome editing (Zn finger, TALEN, and CRISPR/Cas9) by microinjection of RNA and DNA into fertilized
embryos; embryo re-derivation; embryo and sperm cryopreservation; in vitro fertilization; centralized and
monitored cryopreservation storage; and maintenance of generally useful mouse lines including those for
recombination-activation gene expression. The facility uses state-of-the-art laser conditioning of the zona to
facilitate IVF and has intracytoplasmic sperm injection capability on-line to complement the in vitro fertilization
service. Newly developed services during the current funding period include the major expansion of
cryopreservation services with corresponding expansion of the centralized and dedicated cryopreservation
facility, integration of mouse ES microinjection service with a centralized gene knock-out core facility, and
direct genome editing by Zn-finger, TALEN and CRISPR-Cas9 technologies. In the reporting period (10/01/13-
09/30/14), Cancer Center members accounted for 46% of total Facility users and those with peer-reviewed
funding accounted for 42%. This compares favorably with our request for 21% of the total budget from the
CCSG. Additional Institutional support is provided in the form of funding for equipment maintenance and facility
infrastructure upgrades and maintenance.

## Key facts

- **NIH application ID:** 9836817
- **Project number:** 5P30CA016520-44
- **Recipient organization:** UNIVERSITY OF PENNSYLVANIA
- **Principal Investigator:** STEPHEN Aaron LIEBHABER
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $196,501
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9836817

## Citation

> US National Institutes of Health, RePORTER application 9836817, Transgenic and Chimeric Mouse Facility (5P30CA016520-44). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9836817. Licensed CC0.

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