# Statistical Methods for Network-Based Integrative Analysis of CVD Epigenetic Data

> **NIH NIH K01** · UNIVERSITY OF WASHINGTON · 2020 · $137,714

## Abstract

   
DESCRIPTION (provided by applicant): This project involves the development of new statistical methodologies and computational tools for network-based integrative analysis of epigenetic risk factors of cardiovascular diseases (CVD). While the advent of omics data from new technologies has facilitated the study of epigenetic factors, existing methodologies often do not account for complexities of biological data  such as correlations due to interactions of genes/proteins as part of biological pathways  and fail to efficiently integrate diverse omics data
sets  for instance genetic variation, DNA methylation and gene expression. The methodologies proposed in this project, and the software tools that will be developed to implement them, address these shortcomings, and facilitate further research by the biomedical community to gain a better understanding of the underlying biology of CVD, and to develop new diagnostic biomarkers and potential targets for therapies. The proposed methodologies are motivated by the study of epigenetic data from the Multi-Ethnic Study of Atherosclerosis (MESA), and include (i) a network-based pathway enrichment analysis method that incorporates available knowledge of interactions among genes and proteins while complementing and refining such information (Aim 1A), as well as its extension for analysis of multiple types of omics data (Aim 1B), and (ii) an integrative analysis framework to identify associations among gene expression levels and DNA methylation (Aim 2A) and identify common epigenetic factors of multiple CVD phenotypes through integrated analysis of DNA methylation and mRNA expression data (Aim 2B). We will develop efficient and user-friendly software tools for the proposed methods (Aim 3), which will be made freely available to the public after extensive tests using both simulated data, as well as real data from MESA.

## Key facts

- **NIH application ID:** 9838771
- **Project number:** 5K01HL124050-05
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** ALI SHOJAIE
- **Activity code:** K01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $137,714
- **Award type:** 5
- **Project period:** 2015-12-15 → 2021-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9838771

## Citation

> US National Institutes of Health, RePORTER application 9838771, Statistical Methods for Network-Based Integrative Analysis of CVD Epigenetic Data (5K01HL124050-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9838771. Licensed CC0.

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