# Allergen T cell epitopes during the management of peanut allergic disease

> **NIH NIH U19** · BENAROYA RESEARCH INST AT VIRGINIA MASON · 2020 · $4,058

## Abstract

Project Summary
The Gene expression and Systems Immunology (GeSI) Core will provide support for this center by
implementation of advanced analysis of flow and mass cytometry (CyTOF), and bulk and single cell RNA-
sequencing (RNA-seq). Work in the Core to achieve this function will be organized around three technologies.
We will utilize advanced analysis techniques of multi-color flow cytometry and mass cytometry data to
elucidate selected phenotypic properties of peanut allergen-reactive CD4+ T cells. These studies will
complement standard flow and mass cytometry analyses performed in the PI's laboratories in project-specific
experiments. We will use RNA-seq to elucidate full transcriptome phenotypes of bulk populations of
peanut allergen specific CD4+ T cells. Knowledge from these experiments will drive design of better flow
and mass cytometry experiments in the individual projects, and will aid in analysis of single cell RNA-
sequencing (RNA-seq) experiments. We will utilize novel technologies to perform single cell analysis of
transcriptome phenotypes and TCR clonotypes of allergen-specific CD4+ T cells. These experiments will
facilitate understanding of heterogeneity of allergen-specific CD4+ T cells in PA and in determining
mechanism(s) involved in successful OIT. Together, we expect the technologies we provide to play a major
role in completion of the component projects, and in driving improved understanding of immune aspects of PA.
We also anticipate identifying new biomarkers, new targets and better strategies for patient selection in OIT.

## Key facts

- **NIH application ID:** 9840446
- **Project number:** 5U19AI135817-03
- **Recipient organization:** BENAROYA RESEARCH INST AT VIRGINIA MASON
- **Principal Investigator:** Peter S Linsley
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $4,058
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9840446

## Citation

> US National Institutes of Health, RePORTER application 9840446, Allergen T cell epitopes during the management of peanut allergic disease (5U19AI135817-03). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/9840446. Licensed CC0.

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