# Proteomics Shared Resource

> **NIH NIH P30** · OHIO STATE UNIVERSITY · 2020 · $156,099

## Abstract

CORE-013: PROTEOMICS SHARED RESOURCE (PSR)
PROJECT SUMMARY / ABSTRACT
The OSUCCC Proteomics Shared Resource (PSR) provides CCSG investigators access to advanced mass
spectrometry (MS) instrumentation, ancillary instrumentation for sample preparation, and expert staff to enable
proteomic research, including data analysis. Two tenured faculty experts in mass spectrometry and
proteomics, Drs. Michael Freitas (MBCG) and Vicki Wysocki, serve as PSR Director and Senior Faculty
Advisor, respectively. They provide scientific leadership to the PSR with expertise in cancer proteomics,
protein chemistry, bioinformatics, and mass spectroscopy methods development. The PSR offers numerous
proteomics services to users that fall under the categories of protein identification, characterization, and
quantification. The ability to confidently identify proteins is the central role of the PSR. Examples of protein
characterization analysis include: identification of post-translational modifications, alternate splice forms, de
novo protein sequencing and protein-protein interaction analysis. The Specific Aims of the PSR are to: 1)
provide advanced mass spectrometry based proteomics services; 2) provide innovative proteomic data
analytics and bioinformatics platforms; and, 3) provide consultations with investigators on experiment design
and train users on the operation of several self-operated MS instruments within the shared resource. Over the
last grant period, there have been substantial upgrades to equipment in the PSR. Early in the grant period,
three mass spectrometers were acquired with the help of federally funded awards: a Bruker Maxis Q-TOF, a
Bruker UltrafleXtreme MALDI TOF-TOF, and a Bruker AmaZon ion trap with electron transfer dissociation
(ETD). This year, three additional mass spectrometers, two high-end instruments (a Thermo Orbitrap Fusion
and a Bruker 15 T FTICR along with a Thermo Quantiva triple quadrupole for targeted (MRM) analyses) are to
be installed in the PSR with funding from two NIH S10 awards and also OSUCCC and other institutional
support. These state-of-the-art MS instruments will improve services offered to OSUCCC members by
providing significantly higher throughput shotgun proteomics, improved post-translational modification analysis,
improved isotopic fine structure analysis for metabolomics, and greater capacity and data quality. The PSR has
supported 37 OSUCCC members from all five OSUCCC research programs including 1 K24, 1 N01, 8 P01s, 2
P50s, 19 R01s, 8 R21s, 2 RC2s, 1 T32, and 2 U01s. The PSR has also contributed to over 74 OSUCCC
member publications during the last grant period, 11 of which were in publications with a journal impact factor
>10. The future plans for the PSR involve a constant effort to develop and adopt new innovative techniques
and methods for protein analysis, and to acquire state-of-the-art mass spectrometry and chromatography
instrumentation. Specifically, the PSR will fully integrate the 3 recently purchased...

## Key facts

- **NIH application ID:** 9843872
- **Project number:** 5P30CA016058-44
- **Recipient organization:** OHIO STATE UNIVERSITY
- **Principal Investigator:** Michael A. Freitas
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $156,099
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9843872

## Citation

> US National Institutes of Health, RePORTER application 9843872, Proteomics Shared Resource (5P30CA016058-44). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9843872. Licensed CC0.

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