# Cell Engineering Shared Resource

> **NIH NIH P30** · WAKE FOREST UNIVERSITY HEALTH SCIENCES · 2020 · $44,837

## Abstract

CELL AND VIRAL VECTOR LABORATORY SHARED RESOURCE 
Project Summary 
The mission of the Cell and Viral Vector Laboratory (CVVL) Shared Resource is to serve the scientific needs of 
Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) researchers by offering customized 
assistance for specialized cell culture and viral vector production; and by providing quality control for each 
research project. The use of primary and established cultures from cancerous and non-cancerous origins is 
essential for cancer research and requires careful quality control to maintain appropriate phenotype and 
functions. In addition, the use of various viral vectors (including lentivirus, retrovirus and adenovirus) has 
become a critical experimental technique for cancer research to effectively manipulate gene expression and to 
create desired mutations in particular genes. The CVVL also serves as a central site for purchasing and 
distributing specific culture media/reagents, molecular biology kits, and standard laboratory supplies in a cost- 
effective manner for WFBCCC members. The Specific Aims of CVVL are to 1) provide specialized cell culture 
services that support WFBCCC members' research needs, in an efficient and cost-effective manner; 2) 
maintain a cell repository of commonly used cell lines for WFBCCC member use; 3) provide WFBCCC 
members access to specialized equipment not readily available or not cost-effective for individual researchers; 
and 4) serve as a distribution center for critical cell culture and molecular biology reagents. The CVVL is 
extensively utilized by more than 139 Principal Investigators (both WFBCCC members and non-members) 
annually; 63% of services support the research of WFBCCC members. Services are provided to investigators 
on all institutional campuses at substantial cost savings with work conducted under stringent standard 
operating procedures. The expertise of the CVVL Director, Purnima Dubey, Ph.D., and the efforts of two 
technicians (1.5 FTE) provide high-quality cell culture and viral vector production services to researchers at 
WFBCCC. In the future, the CVVL plans to extend the viral production service by expanding its cutting-edge 
viral vector systems.

## Key facts

- **NIH application ID:** 9848532
- **Project number:** 5P30CA012197-45
- **Recipient organization:** WAKE FOREST UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Ravi N Singh
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $44,837
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9848532

## Citation

> US National Institutes of Health, RePORTER application 9848532, Cell Engineering Shared Resource (5P30CA012197-45). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9848532. Licensed CC0.

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