# Animal Models Core

> **NIH NIH U19** · DUKE UNIVERSITY · 2020 · $629,514

## Abstract

The overall goal of the Animal Models Core is to support all programs of the IPCAVD by performing
immunization studies in CH58 unmutated common ancestor (UCA) VH + VL knock-in (KI) mice and rhesus
macaques, with ALVAC-C vCP2438 prime and modified mRNA encoding trivalent mosaic gp120s for induction
of polyfunctional anti-HIV-1 non-neutralizing antibodies (NNAbs) (Project 1, Aims 2 and 3), and by overseeing
toxicity studies using CGMP clinical trials material (CTM) mRNAs in collaboration with Project 2. This work will
be facilitated by recent innovative advances made in a number of complementing areas, including the
development of novel SHIV constructs expressing transmitted/founder HIV-1 envelopes, well-tolerated
adjuvants that substantially increase the immunogenicity of HIV-1 envelope protein immunogens and induce T
follicular helper cell (Tfh) responses, technology for reproducibly generating envelope-specific monoclonal
antibodies from immunized macaques, and use of the new CH58 VH and VL knock-in mouse that expresses
one of the NNAb ADCC-mediating antibodies from RV144, CH58 V2 antibody. The Specific Aims of this
Animal Models Core will include:
Aim 1. Perform immunization studies in CH58 unmutated common ancestor (UCA) homozygous
(hom)/heterozygous (het) VH + VL knock-in (KI) mice ALVAC-C vCP2438 prime and modified mRNA
encoding trivalent mosaic gp120s in Project 1, Aim 2.
Aim 2. Perform immunization studies with ALVAC-C vCP2438 prime and boosting with and boosting
with ALVAC-C plus modified mRNA encoding trivalent mosaic gp120s mRNAs in rhesus macaques
(RMs) (Project 1, Aim 3).
Aim 3. Perform immunization studies in CH58 UCA hom/het VH + VL KI mice to confirm CGMP clinical
trials materials immunogenicity, and oversee toxicity studies of ALVAC-C + CGMP mRNAs in
collaboration with Project 2.

## Key facts

- **NIH application ID:** 9849191
- **Project number:** 5U19AI142596-02
- **Recipient organization:** DUKE UNIVERSITY
- **Principal Investigator:** Barton F. Haynes
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $629,514
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9849191

## Citation

> US National Institutes of Health, RePORTER application 9849191, Animal Models Core (5U19AI142596-02). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9849191. Licensed CC0.

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