# Epithelial Cell and Mucosal Immunology

> **NIH NIH P30** · BOSTON CHILDREN'S HOSPITAL · 2020 · $196,597

## Abstract

The Epithelial Cell and Mucosal Immunology Core (Core C) provides the infrastructure, biologic resources, 
expertise, and training for innovative and cross-disciplinary research on epithelial biology and immune 
function in mucosal surfaces and solid organs of the GI tract. 
Specific activities and services include: 
 · Polarized Epithelial Cell Culture and Genetic Transformations 
 · nCounter NanoString RNA Analysis 
 · FACs 7- or 12-Color Analysis Of Cultured Cells or Tissue/Organoid-derived Primary Cells 
 · Transepithelial Electrophysiology of live cells, cell monolayers, and tissues 
 · Gastrointestinal Organoid Derivation and Culture 
 · Specialized Equipment, Reagents, Training, and Consultation for recombinant protein expression and 
 purification, including small molecules, specialized and unique epithelial cell lines, expression profiling 
 using Bio-Plex MAGPIX multiplex reader 
 · Research and Development of live cell ratio fluorescence imaging of ion and solute transients, and 
 FRAP analysis of membrane molecules 
A total of 27 HDDC members and 20 non-Members used Core C in the last grant cycle as measured in 
billable hours of service. 8 HDDC Members used Core C services in every quarter. The Core recorded 
>4000 billable hours/year. 
45 Members and Associate Members anticipate use of Core C in the next grant cycle. All services will be 
utilized.

## Key facts

- **NIH application ID:** 9850242
- **Project number:** 5P30DK034854-35
- **Recipient organization:** BOSTON CHILDREN'S HOSPITAL
- **Principal Investigator:** WAYNE I LENCER
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $196,597
- **Award type:** 5
- **Project period:** — → 2021-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9850242

## Citation

> US National Institutes of Health, RePORTER application 9850242, Epithelial Cell and Mucosal Immunology (5P30DK034854-35). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9850242. Licensed CC0.

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