# Analysis of lamin-chromatin interactions and their regulation of chromosome organization and gene expression

> **NIH NIH R01** · CARNEGIE INSTITUTION OF WASHINGTON, D.C. · 2020 · $492,128

## Abstract

ABSTRACT
The goal of our research is to determine the mechanisms by which the lamin isoform meshworks comprising
the nuclear lamina (NL) organize chromatin in 3D and regulate gene expression. The experimental plan builds
on recently developed techniques for determining lamin meshwork structures at nanoscale resolution using
3D-SIM combined with computational image analysis. This structural approach will be coordinated with studies
of the biochemical/molecular interactions of each lamin isoform with chromatin using our recently developed
HiLands model, which is the first to focus on the role of lamins in regulating chromatin organization.
Throughout the proposed studies we will take advantage of our collection of single, double and triple lamin
knockout mouse cells. Specifically we will elucidate the steps involved in the assembly of lamin isoform
meshworks within the NL and determine how the assembly state of lamins impacts the 3D organization of
chromatin and gene expression. These studies will be accompanied by a multi-pronged approach employing
Hi-C, 4-C, epigenomics, transcriptomics, chromosome painting and FISH, the latter combined with 3D SIM-
based immunostaining, to determine whether lamins regulate gene expression by maintaining 3D chromatin
folding, enhancer-promoter interactions, and epigenetic modifications.

## Key facts

- **NIH application ID:** 9850602
- **Project number:** 5R01GM106023-08
- **Recipient organization:** CARNEGIE INSTITUTION OF WASHINGTON, D.C.
- **Principal Investigator:** ROBERT D GOLDMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $492,128
- **Award type:** 5
- **Project period:** 2013-06-01 → 2021-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9850602

## Citation

> US National Institutes of Health, RePORTER application 9850602, Analysis of lamin-chromatin interactions and their regulation of chromosome organization and gene expression (5R01GM106023-08). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9850602. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*