# Transgenic & ES Cell

> **NIH NIH P30** · WASHINGTON UNIVERSITY · 2020 · $76,250

## Abstract

ABSTRACT: Transgenic and ES Cell Core
The Transgenic and ES Cell Core of the Washington University Diabetes Research Center (DRC) provides
services for efficient and cost-effective development of genetically altered mouse models to elucidate the
pathogenesis of diabetes and related metabolic disorders. Importantly, in vivo models allow analysis of gene
function in the context of complex intercellular and integrative systemic responses needed for the study of
diabetes, a polygenic disorder involving multiple tissue types and environmental influences. Current molecular
genetic techniques allow spatially and temporally restricted analysis of gene effects (both inactivation and over-
expression) in mice, leading to previously unprecedented creativity and analytical resolution. The concepts
involved in generating transgenic (Tg) or knockout/knockin (KO/KI) animals are straightforward. However, it is
impractical and too expensive for most labs to maintain the requisite equipment, facilities, and highly skilled
personnel for making genetically altered mice. Given the substantial demand by the Washington University
community for assistance in the generation of these mouse models, the DRC Transgenic and ES Cell Core will
continue to provide the necessary advice, expertise, facilities, equipment, and personnel to efficiently generate
Tg and KO/KI mice for DRC members using state-of-the-art techniques, such as CRISPR/Cas9 nuclease-
mediated genome editing in ES cells and in mouse zygotes. The Core will also assist in propagating poorly
breeding animals, and in achieving transient Cre or FLP recombinase expression in zygotes by microinjection
of expression plasmids. By making specialized, state-of-the-art services accessible to DRC investigators, the
Core facilitates translation of basic research advances toward improvements in the diagnosis, treatment and
cure of diabetes.

## Key facts

- **NIH application ID:** 9851904
- **Project number:** 5P30DK020579-43
- **Recipient organization:** WASHINGTON UNIVERSITY
- **Principal Investigator:** JEFFREY H MINER
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $76,250
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9851904

## Citation

> US National Institutes of Health, RePORTER application 9851904, Transgenic & ES Cell (5P30DK020579-43). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9851904. Licensed CC0.

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