# Applied Research Project

> **NIH NIH P40** · JACKSON LABORATORY · 2020 · $758,473

## Abstract

PROJECT SUMMARY/ABSTRACT – Applied Research Project
The overall goal of the SMSR is to provide the tools and resources for complex trait analysis in mice. The
ultimate goal of complex trait analysis is the identification of specific genes and pathways, which requires proof
of causation. Causation is best demonstrated through replacement of specific allelic variants in their original
context, recapitulation of allelic variants on an inbred strain background, and/or biochemical assays in
engineered cell lines or stem cells. To advance the study of complex traits novel mouse populations, like the
Diversity Outcross (DO), have been developed to allow for ultra high-resolution mapping. The small QTL that
can be obtained through application of the DO population comes at an expense because individual DO
genomes cannot be reproduced through standard husbandry or assisted reproductive technologies, and
therefore individual DO genomes cannot be genetically engineered. The overall goal of the proposed applied
research project is to explore methods for stabilization of extreme DO genomes through cloning. These
methods include the derivation of iPS cells from adult fibroblasts and the production of 100% iPS cell derived
chimeras. The establishment of these protocols within the SMSR will provide the foundation for a new service
whereby the reagents for DO genome stabilization, candidate gene testing and proof of causation can be
available through the resource.

## Key facts

- **NIH application ID:** 9851960
- **Project number:** 5P40OD011102-20
- **Recipient organization:** JACKSON LABORATORY
- **Principal Investigator:** LAURA G REINHOLDT
- **Activity code:** P40 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $758,473
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9851960

## Citation

> US National Institutes of Health, RePORTER application 9851960, Applied Research Project (5P40OD011102-20). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/9851960. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*