# Ambystoma Genetic Stock Center

> **NIH NIH P40** · UNIVERSITY OF KENTUCKY · 2020 · $38,083

## Abstract

APPLIED RESEARCH COMPONENT - PROJECT SUMMARY
This component addresses the significance and need to develop a protocol for cryopreserving axolotl
germplasm. The AGSC will work with the Aquatic Germplasm and Genetics Research Center at Louisiana
State University (LSU) to develop a reproducible protocol for cryopreserving axolotl sperm. Early results are
presented to show that the AGSC has already initiated collaborative studies with the AGGRC to assess axolotl
sperm viability and motility after overnight transport from Lexington to Louisiana. Building upon this work, the
AGSC will ship axolotl sperm to the AGGRC to identify optimal conditions for cryopreservation. After a working
protocol has been established, AGSC staff will visit the AGGRC to learn how to assess sperm quality and
perform the cryopreservation procedure. Additionally, AGGRC staff will help the AGSC develop a repository
strategy for community-wide management of axolotl sperm. Upon returning to Lexington, KY, AGSC staff will
implement the protocol, examine the viability of frozen sperm, and then use cryopreserved sperm to re-derive
axolotl stocks. Accomplishment of this objective will allow for more efficient management of axolotl stocks
within the AGSC and the broader axolotl community

## Key facts

- **NIH application ID:** 9853492
- **Project number:** 2P40OD019794-06
- **Recipient organization:** UNIVERSITY OF KENTUCKY
- **Principal Investigator:** Stephen Randal Voss
- **Activity code:** P40 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $38,083
- **Award type:** 2
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9853492

## Citation

> US National Institutes of Health, RePORTER application 9853492, Ambystoma Genetic Stock Center (2P40OD019794-06). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/9853492. Licensed CC0.

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