# Flow Cytometry Core

> **NIH NIH P30** · UNIVERSITY OF VIRGINIA · 2020 · $83,511

## Abstract

FLOW CYTOMETRY CORE – PROJECT SUMMARY/ABSTRACT 
The Flow Cytometry Core (FCC), in existence since 1982, is a state-of-the-art shared resource that provides 
Cancer Center investigators access to high quality, cost effective flow cytometry services and technology. The 
facility occupies approximately 1850 sq. ft. within the Cancer Center wing in Jordan Hall. By providing a variety 
of instrumentation and the scientific expertise necessary to effectively use these technologies, the facility 
serves to enhance the scope and quality of cancer research performed at the University. The FCC 
incorporates a variety of technologies into its services based on the needs of the Cancer Center investigators. 
The instrumentation available is compatible wide array of flow cytometric applications such as subpopulation 
identification/quantification, molecular detection, measurement of DNA and RNA content, apoptosis, 
transcriptional activity, cell viability, membrane potential, cell sorting, and bead based immunoassays. The 
FCC provides cell sorting in enclosed Biosafety Cabinets to allow for the safe sorting of primary human 
samples, as well as retro/lentivirally transfected cells. The sorters are capable of up to 17 parameters, with six- 
way, single cell and index sorting capabilities for downstream single cell genomics assays. The recent addition 
of the CyTOF2 mass cytometer, through joint funding from the President's office and several 
departments/centers, allows major advancements in the evaluation of highly dimensional biological systems to 
unravel the complexity of cancers and their associated immune responses. The imaging flow cytometry 
services provide additional capabilities for evaluating a variety of measurements at the single cell level, with 
high throughput and statistical robustness. Direct collaborations with Cancer Center investigators have 
produced novel methods and approaches for studying cancer that have been published. The services and 
expertise offered by the FCC play a key role in the study of many types of cancer, as well as the immune 
responses to cancers. The FCC staff is highly qualified with many years of experience and are all certified by 
the International Cytometry Certification Exam (ICCE). In addition to providing training on the instrumentation, 
FCC staff provide consultation in experimental design, sample preparation and data analysis. The facility has 
recently added a bio-analysis specialist to assist Cancer Center investigators with unraveling the complexity of 
the data generated in the core. Specialized training classes are offered for those researchers who wish to 
better understand the principles and techniques employed in this technology and prefer to directly acquire 
and/or analyze their own samples. Researchers have the option, once trained, of performing their own analysis 
or they can choose to utilize the expertise of the FCC's staff to run their samples for them.

## Key facts

- **NIH application ID:** 9854915
- **Project number:** 5P30CA044579-29
- **Recipient organization:** UNIVERSITY OF VIRGINIA
- **Principal Investigator:** Thomas P. Loughran
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $83,511
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9854915

## Citation

> US National Institutes of Health, RePORTER application 9854915, Flow Cytometry Core (5P30CA044579-29). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9854915. Licensed CC0.

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