# Mechanisms of impaired mucociliary transport in CF airway disease

> **NIH NIH K08** · UNIVERSITY OF IOWA · 2020 · $160,164

## Abstract

PROJECT SUMMARY/ABSTRACT
Impaired mucociliary transport (MCT) is a common feature of multiple airway diseases, including cystic fibrosis
(CF). Effective MCT requires functional cilia and mucus. The mechanical properties of mucus influence MCT.
In CF, loss of bicarbonate transport in the airway epithelium decreases the pH of the airway surface liquid
(ASL). The acidic ASL changes the electrostatic interactions of mucus, making the mucus more viscous. This
defect is present at birth prior to the onset of infection and inflammation. Newborn CF pigs also have impaired
MCT. We predict that the viscous mucus in newborn CF pigs gives rise to defective MCT.
In the current application, the candidate will probe the biophysical properties of freshly isolated porcine airway
mucus using agents that alter mucus electrostatic interactions. He will assess the biophysical properties using
passive microrheology. Concomitantly, he will measure MCT in newborn CF pigs using computed tomography
(CT) and correlate how altered biophysical properties of mucus influence MCT.
The candidate's long-term career goal is to become a leader in the fields of airway biology and airway disease.
He plans to advance both fields by utilizing advanced imaging techniques and porcine models. The selection
of the porcine model is particularly relevant because the airway anatomy and physiology more closely
resemble humans than traditional rodent models.
In the current K08 application, the candidate will gain multiple intellectual, professional and technical skills to
foster his development into an independent and successful physician scientist. He has an outstanding mentor
and advisory committee that will guide him in learning passive microrheology of mucus and whole animal CT.
This topic is highly significant as mucus biophysical properties influence MCT, and thus the candidate's work
can shed light on basic biology and also potential therapeutics. In addition, the candidate will continue to
develop his manuscript and grant writing abilities through reviewing of manuscripts and grants under Dr.
Welsh's guidance. He will also take coursework in biomedical and biochemical engineering to advance his
understanding of the complex principles of rheology and imaging analyses. Lastly, the candidate will continue
to cultivate his own mentoring skills through learning how to manage timelines and budgets, as well as train
junior scientists.
In summary, this award will greatly enrich the candidate's career by providing him with additional tools
necessary to become an independent and successful physician scientist that contributes to the betterment of
human health.

## Key facts

- **NIH application ID:** 9855052
- **Project number:** 5K08HL135433-04
- **Recipient organization:** UNIVERSITY OF IOWA
- **Principal Investigator:** Mahmoud Abou Alaiwa
- **Activity code:** K08 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $160,164
- **Award type:** 5
- **Project period:** 2017-02-01 → 2022-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9855052

## Citation

> US National Institutes of Health, RePORTER application 9855052, Mechanisms of impaired mucociliary transport in CF airway disease (5K08HL135433-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9855052. Licensed CC0.

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